Proteolytic enzymes in green wheat leaves I. Isolation on DEAE-cellulose of several proteinases with acid pH optima1
- 1 May 1978
- journal article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 19 (3) , 491-500
- https://doi.org/10.1093/oxfordjournals.pcp.a075618
Abstract
Several peptide hydrolases have been isolated in a partially purified form from green wheat leaves by a combination of gel chromatography and chromatography on DEAE-cellulose. These hydrolases all degrade haemoglobin and other substrates tested at an acid pH. Three of the enzymes have been identified as proteinases by measurement of the total N: α-amino N ratio of the degradation products. These three enzymes have a similar optimum assay pH of about 4.5 with haemoglobin as substrate, but differ markedly in their stability at high temperatures.Keywords
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