Isolation and characterization of high-mobility-group proteins from maize
- 1 October 1991
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 185 (3) , 350-355
- https://doi.org/10.1007/bf00201055
Abstract
Chromosomal nonhistone high-mobility-group (HMG) proteins were purified from nuclei of maize (Zea mays L. cv. A619) endosperm and leaf tissue. Tissuespecific differences were observed in their polypeptide patterns, in in-vitro phosphorylation experiments with a casein-kinase type II, and by Western blot analysis with antisera against different HMG proteins. Gelfiltration chromatography demonstrated that maize HMG proteins occur as monomers. By measuring the capacity of the HMG proteins to bind to the 5′ flanking region of a zein gene, the sensitivity of the proteins to different temperatures, salt concentrations and pH values was determined.Keywords
This publication has 33 references indexed in Scilit:
- High mobility group proteins 1 and 2 function as general class II transcription factorsBiochemistry, 1990
- Expression of mRNAs encoding mammalian chromosomal proteins HMG-I and HMG-Y during cellular proliferationExperimental Cell Research, 1990
- Conjugates of Synthetic Lymphocyte-Activating Lipopeptides with Segments from HIV Proteins Induce Protein-Specific Antibody FormationBiological Chemistry Hoppe-Seyler, 1990
- HMG I-like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters.Plant Cell, 1990
- Tissue specificity of nucleo‐cytoplasmic distribution of HMG1 and HMG2 proteins and their probable functionsEuropean Journal of Biochemistry, 1989
- A nuclear casein type II kinase from maize endosperm phosphorylating HMG proteinsBiochemical and Biophysical Research Communications, 1989
- High mobility group protein 1 preferentially conserves torsion in negatively supercoiled DNABiochemistry, 1989
- The intracellular distribution and function of the high mobility group chromosomal proteinsExperimental Cell Research, 1985
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970