The effect of amiloride and benzimidazoleguanidine added to the inside medium on electrolyte pathways in the frog skin glands

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Abstract
Serosal amiloride inhibits Na+, K+and Cl‐efflux and reduces short‐circuit current and transepithelial conductance in noradrenaline‐stimulated frog skin in which the sodium channels in the apical membrane are blocked by amiloride. BIG (benzimidazoleguanidine) inhibits Na+and K+efflux and reduces the short‐circuit current. In some skins BIG decreased and in others it increased Cl‐efflux and conductance. The variable response appears to be due not only to inhibition of salt extrusion from the gland cells but also to activation of Cl‐transport through the mitochondria‐rich cells, since it was shown that BIG could increase Cl‐efflux in gland‐free preparations. Different target mechanisms in the gland cells where the two substances may exert their effect are discussed.