Pineapple‐tissue based bioelectrode for the determination of hydrogen peroxide

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Abstract
A flow‐injection analysis (FIA) system for the determination of hydrogen peroxide was developed by utilizing a pineapple‐tissue‐based modified carbon paste electrode. Pineapple stem core tissue contains peroxidase, which is not available commercially in the pure isolated form. Hydrogen peroxide concentrations were determined in the presence of O‐phenylenediamine as a hydrogen donor at a potential of −200 mV. The detection limit (S/N = 3) for hydrogen peroxide using this tissue electrode is 2.1 μM with a relative standard deviation of 0.44% (n = 12). The tissue‐modified carbon paste electrode is stable for up to 20 days with no significant activity changes.