γδ T lymphocyte responses to HIV

Abstract
Natural immunity may be involved in controlling viral spread in hosts infected with HIV. A panel of γδ T cell receptor‐positive lymphocyte clones was isolated from the peripheral blood of healthy HIV donors and tested for anti‐HIV cytotoxic responses. Twelve of 30 (40%) Vγ9+Vδ2+ T cell clones, but none of seven Vδ1+ T cell clones, displayed lytic activity against HIV‐infected cells. The Vγ9+/Vδ2+ clones cytotoxic for HIV‐infected cells also lysed Daudi cells. However, not all Vγ9+/Vδ2+ clones which lysed Daudi targets had the capacity to lyse HIV‐infected cells. Some of the γδ T cell clones were also investigated for potential proliferative responses to HIV‐infected cells. One Vγ9+/Vδ2+ T cell clone (ME8‐7) and one Vδ1+ T cell clone (ME18‐2) demonstrated proliferative responses toward HIV‐infected cells. Another Vγ9+/Vδ2+ clone (VM39) proliferated in response to cell‐free HIV. Taken together, these results provide direct evidence of anti‐HIV γδ T cell responses in healthy, HIV persons.

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