Microbiological Aspects of the Batch Preparation of Replacement Fluid for Hemofiltration

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Abstract
A batch preparation system for manufacturing the replacement fluid for hemofiltration was investigated by microbiological methods. The elements of the pretreatment system of tap water (depth filters, charcoal and softener) increased the bacterial content of the water. Weekly filter change reduced the contamination. The ultrafilter protection of the reverse osmosis (RO) reduced the RO product water bacterial count by log 2. However, the batch contamination was not influenced by the ultrafilter protection of the RO. The system is capable of producing sterile and negative limulus amebocyte lysate (Pyrogent®, Mallinckrodt) reacting replacement fluid for hemofiltration.

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