STRUCTURE AND FUNCTION OF E. coli RIBOSOMES, VIII. COLD-SENSITIVE MUTANTS DEFECTIVE IN RIBOSOME ASSEMBLY
- 1 June 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 63 (2) , 384-391
- https://doi.org/10.1073/pnas.63.2.384
Abstract
E. coli mutants that fail to grow at 20°C were isolated, and three such mutants were studied in detail. Two of these are defective in 50 S ribosome biosynthesis at low temperatures and accumulate 43 S and 32 S particles, respectively. The third mutant produces reduced amounts of 50 S ribosomes and accumulates 21 S particles at low temperatures. The 43 S and 32 S particles contain 23 S RNA and appear to be precursors of 50 S ribosomes. The 21 S particles contain 16 S RNA. The mutation that causes accumulation of 32 S particles is linked to the spc locus. Isolation and characterization of coldsensitive mutants should provide a fruitful approach to the study of in vivo ribosomal assembly.Keywords
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