Repetitive DNA of Higher Plants

1 April 1974

journal article
Published by Oxford University Press (OUP) in Journal of Experimental Botany

Vol. 25 (2) , 320-329
https://doi.org/10.1093/jxb/25.2.320

Abstract

Jerusalem artichoke DNA, extracted from resting rhizomes, was analysed by the reassociation kinetics of sheared denatured fragments. Various fractions were isolated according to their reassociation rates. The slowest fractions consist of unrepeated or single-copy DNA which makes up 55 per cent of the total DNA. The total sequence length of the haploid genome is estimated as corresponding to 0.23×10¹² daltons. Among the fractions of repeated DNA isolated, two were relatively homogeneous with an average complexity of 2×10⁸ and 10⁹ daltons respectively.

Keywords

RESTING RHIZOMES
ARTICHOKE DNA
JERUSALEM ARTICHOKE
KINETICS
DALTONS
REASSOCIATION

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