Proton and nitrogen-15 resonance assignments of oxidized flavodoxin from Anacystis nidulans with 3D NMR
- 1 August 1991
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 30 (31) , 7718-7730
- https://doi.org/10.1021/bi00245a008
Abstract
Proton and nitrogen-15 sequence-specific nuclear magnetic resonance assignments have been determined for recombinant oxidized flavodoxin from Anacystis nidulans (169 residues, Mr 19,048). Assignments were obtained by using 15N-1H heteronuclear three-dimensional (3D) NMR spectroscopy on a uniformly nitrogen-15 enriched sample of the protein, pH 6.6, at 30 degrees C. For 165 residues, the backbone and a large fraction of the side-chain proton resonances have been assigned. Medium- and long-range NOE's have been used to characterize the secondary structure. In solution, flavodoxin consists of a five-stranded parallel beta sheet involving residues 3-9, 31-37, 49-56, 81-89, 114-117, and 141-144. Medium-range NOE's indicate the presence of several helices. Several 15N and 1H resonances of the flavin mononucleotide (FMN) prosthetic group have been assigned. The FMN-binding site has been investigated by using polypeptide-FMN NOE's.Keywords
This publication has 34 references indexed in Scilit:
- Concerted two-dimensional NMR approaches to hydrogen-1, carbon-13, and nitrogen-15 resonance assignments in proteinsBiochemistry, 1989
- Flavodoxin from Anabaena 7120: uniform nitrogen-15 enrichment and hydrogen-1, nitrogen-15, and phosphorus-31 NMR investigations of the flavin mononucleotide binding site in the reduced and oxidized statesBiochemistry, 1988
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteinsBiochemical and Biophysical Research Communications, 1983
- Two-dimensional double quantum 1H NMR spectroscopy of proteinsBiochemical and Biophysical Research Communications, 1983
- Structure of oxidized flavodoxin from Anacystis nidulansJournal of Molecular Biology, 1983
- Sequential resonance assignments in protein 1H nuclear magnetic resonance spectraJournal of Molecular Biology, 1982
- A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromoleculesBiochemical and Biophysical Research Communications, 1980
- Structure of the semiquinone form of flavodoxin from Clostridium MPJournal of Molecular Biology, 1977
- Isolation of two proteins with chloroplast ferredoxin activity from a blue-green algaBiochemical and Biophysical Research Communications, 1965