Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (24) , 10373-10383
- https://doi.org/10.1093/nar/17.24.10373
Abstract
The synthesis of the deoxynucleoside derived from the base P, 6H, 8H-3,4-dihydro-pyrimido[4,5-c] [1,2]oxazin-7-one, 2, and its introduction by established phosphoramidite and H-phosphonate chemistry into oligonucleotides is described. The melting transition temperatures (Tm) of a range of heptadecamer duplexes containing P/A and P/G base-pairs are compared with corresponding ones having N4-methoxycytosine (M) 1 and mismatched normal bases. P/A and P/G pairs allow closely similar duplex stabilities and have the potential to reduce the multiplicity of probes and primers based on amino acid sequence by removing the T/C degeneracy.This publication has 13 references indexed in Scilit:
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