Epstein-Barr virus viral capsid antigen titer by immunofluorescence with microplates: new semiautomated method based on the microtiter system

Abstract
A semiautomated method of an indirect immunofluorescence technique for the titration of antibodies directed against viral capsid antigens of Epstein-Barr virus was developed with Microtiter system units. By this method, a technician is able to titrate some hundred samples daily. The technique is safe, easy and reproducible. The various procedures are described, and the sensitivity of the test is discussed.