Effect of Seasonal Photoperiod on Acute Toxic Responses of Juvenile Rainbow Trout (Salmo gairdneri) to Pulpmill Effluent

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Abstract
Acute toxic responses to bleached kraft pulpmill effluent were compared for groups of underyearling rainbow trout (Salmo gairdneri) reared under constant temperature and either a summer (16L:8D) or winter (8L:16D) photoperiod. Toxicity tests included a 96-h LC50 bioassay, a 4.5-h sealed-jar test, an 18-h temperature tolerance test, and a 4-h blood-sugar stress bioassay. For all tests the threshold-effect concentration of effluent was not affected by photoperiod. Median effective concentrations for the blood-sugar, temperature tolerance, and sealed-jar tests were, respectively, < 0.2, 0.4, and ~1.4 times the 96-h LC50 value for each fish group. Key words: Salmo gairdneri, photoperiod, BKME, acute toxicity tests, LC50, blood sugar, temperature tolerance, residual oxygen

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