Mitosis and Binucleation in Mast Cells of the Rat2

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Abstract
Mast cells in spreads of mesentery from 16 normal rats were colored with the Feulgen reaction and methyl green to permit identification of these cells and determination of their index of mitosis, deoxyribonucleic acid (DNA) doubling, and binucleation in relation to age and growth of the rats. Experimental induction of mitosis of mesenteric mast cells was accomplished by injection of talc suspension into the peritoneal cavity of additional rats. The results of these studies indicated that mesenteric mast cells undergo considerable mitotic activity and that they divide as a function of the growth of the tissue comprising them, whether the growth be natural or experimentally induced. The findings indicated that 1) the index of mitosis and index of DNA doubling of the mast cells and the index of growth of the rats followed similar decreasing trends as the rats advanced in age from 10 to 120 days; 2) the indexes of DNA doubling and binucleation of mast cells increased in relation to the index of mitosis as the three indexes decreased, which suggested that, as the rats became older and their growth subsided, the initiation of karyokinesis and cytokinesis of some mast cells was somehow delayed; 3) the process of binucleation, or failure of dividing cells to undergo cytokinesis, was a naturally occurring process in mesenteric mast cells of the rat; 4) paired mast cells, resulting from divisions of single mast cells, were separated rapidly in young rats and slowly in older rats by growth of mesentery between them.

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