Somatic Embryos and Plantlets from an Ancient Clone of the Grapevine (cv. Cabernet-Sauvignon) by Apomixisin vitro
- 1 October 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 27 (5) , 1022-1030
- https://doi.org/10.1093/jxb/27.5.1022
Abstract
Plantlets were produced by induction of nucellar embryony (apomixis) in isolated unfertilized ovules of a non-apomictic plant—the grapevine (Vitis vinifera L. cv. Cabernet-Sauvignon). Ovules grown in liquid culture with benzyladenine (5–10 μM) and β-naphthoxyacetic acid (5–25 μM) formed a nucellar callus which gave rise to somatic embryos. For growth of plantlets the embryos were transferred to an agar medium containing gibberellic acid (1 μM) and 2-isopentenyladenine (5 μM). This is the first report of somatic embryo formation in a cultivar of a temperate fruit.Keywords
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