Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations
- 10 August 2004
- journal article
- letter
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 20 (19) , 7901-7903
- https://doi.org/10.1021/la049002d
Abstract
We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier transform infrared spectra of such self-assembled (sub)monolayers deliver important structural information of the membrane proteins and are suited to screen the function of cellular receptors.Keywords
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