Structure and expression of cloned murine IFN-α genes
Open Access
- 11 February 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (3) , 555-573
- https://doi.org/10.1093/nar/11.3.555
Abstract
The mouse has an interferon-a (MuIFN-a) gene family containing at least four, and likely more than ten members. A segment of mouse chromosomal DNA and cDNAs encoding murine alpha IFNs have been cloned, and the sequence of two MuIFN-a DNAs determined. No intron was found in the chromosomal gene. The two coding sequences produced biologically active IFN when expressed in monkey cells under the control of an SV40 promoter, and in E.coli under the control of the ampicillinase promoter. MuIFN-al had no detectable activity on human cells, while MuIFN-a2 was 201 as active on human as on mouse cells.Keywords
This publication has 54 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- DNA Sequence of Two Closely Linked Human Leukocyte Interferon GenesScience, 1981
- The structure of eight distinct cloned human leukocyte interferon cDNAsNature, 1981
- The structure of one of the eight or more distinct chromosomal genes for human interferon-αNature, 1980
- Synthesis in E. coli of a polypeptide with human leukocyte interferon activityNature, 1980
- Structure and genomic organization of the mouse dihydrofolate reductase geneCell, 1980
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- RAISING ANTIBODIES TO HUMAN LEUKOCYTE INTERFERONActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1975