THE EFFECT OF ULTRASONIC WAVES UPON KLEBSIELLA PNEUMONIAE, SACCHAROMYCES CEREVISIAE, MIYAGAWANELLA FELIS, AND INFLUENZA VIRUS A

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Abstract
In a series of expts. using a quartz crystal generator (700,000 cycles/ sec.) of ultrasonic energy (max. output 765 watts) the following factors were found to have no effect, within the range tested, upon the mortality rate of K. pneumoniae treated with ultrasonic waves: age of the culture being treated, concn. of cells in the suspension being treated, and distance between the bottom of the container and the crystal. Type of container used for the treatment, efficiency of the cooling of the suspension being treated, intensity of the ultrasonic energy and type of suspending medium produced differences in the mortality rate. S. cerevisiae was somewhat more resistant to ultrasonic waves than K. pneumoniae M. felis and influenza A virus (PR8 strain) were inactivated by ultrasonic waves. Electron micrographs of these microorganisms and viruses after ultrasonic treatment are included.