Identification of Fibrinogen Derivatives in Plasma Samples
- 1 January 1972
- journal article
- schattauer gmbh
- Published by Georg Thieme Verlag KG in Thrombosis and Haemostasis
- Vol. 27 (03) , 610-618
- https://doi.org/10.1055/s-0038-1649400
Abstract
The observation of fibrinogen derivatives with a molecular weight higher than the parent molecule in human cases of DIC initiated the present methodological study. These derivatives were identified by the following methods : 2.5 M β-alanine precipitation of the plasma samples, PAA gel electrophoresis, intra gel immunoprecipitation and agarose gel chromatography. In the plasma of a patient with severe eclampsia and laboratory signs of DIC two derivatives with a molecular weight higher than that of fibrinogen were identified according to their relative electrophoretic mobilities: 0.18 and 0.28 × 10−5 cm2/V × sec (fibrinogen: 0.43 × 10−5 cm2/V × sec). Electrophoretic studies in the presence of 5 M urea indicated that the 0.28 derivative is a complex probably formed by fibrinogen and a fibrin monomer. * DFG- Research Trainee, SFB 51.Keywords
Funding Information
- DFG (2/Gra)
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