The Structure of GABPα/β: An ETS Domain- Ankyrin Repeat Heterodimer Bound to DNA

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Abstract
GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The α subunit contains a DNA-binding domain that is a member of the ETS family, whereas the β subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPα/β ETS domain–ankyrin repeat heterodimer bound to DNA was determined at 2.15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPα ETS domain binds to its core GGA DNA-recognition motif.