New Method for Detection of Mouse Mammary Tumor Virus. II. Effect of Administration of Lactating Mammary Tissue Extracts on Incidence of Hyperplastic Mammary Nodules in BALB/cCrgl Mice23
- 1 July 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 31 (1) , 75-89
- https://doi.org/10.1093/jnci/31.1.75
Abstract
One- to 2-day old male and female BALB/cCrgl mice received intraperitoneal injections of lactating mammary tissue extracts from various MTV-free and MTV-carrying strains of mice. Control mice of both sexes were given injections of saline. Beginning at 36 days of age, intact female and gonadectomized male mice were treated daily for 90 days with a “noduligenic” hormone combination containing estradiol plus deoxycorticosterone acetate. The animals were killed either on the 22d or on the 36th day after the last hormone injection. Hyperplastic mammary nodules were absent from control mice given saline, and from all but one of the mice given injections of tissue extracts from MTV-free strains. These nodules were present, however, in the mammary glands of 39 of the 46 mice with injections of tissue extracts from MTV-carrying strains. These experiments thus demonstrate that the hormonal induction of rapid nodule formation in BALB/cCrgl mice is a suitable method for the qualitative detection of the mouse mammary tumor virus.Keywords
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