Inactivation of Benzodiazepine Binding Sites by N‐Ethylmaleimide
- 1 June 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 38 (6) , 1768-1770
- https://doi.org/10.1111/j.1471-4159.1982.tb06661.x
Abstract
The benzodiazepine receptors of bovine brain membranes were identified by the specific binding of radiolabeled [3H]diazepam [an antianxiety agent]. Pretreatment of membranes with N-ethylmaleimide causes a dose- and time-dependent decrease of 45-60% in the number of binding sites. No decrease occurs when membranes are pretreated with N-ethylmaleimide before administration or in the presence of diazepam. [3H]Diazepam binding to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.Keywords
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