Vasoactive Intestinal Polypeptide Influence on Lower Urinary Tract Smooth Muscle from Human and Pig

Publisher Website
Google Scholar
Abstract
The influence of vasoactive intestinal polypeptide [VIP] on detrusor, trigone, bladder neck and urethral smooth muscle from human and pig was investigated in vitro. VIP reduced the tension and amplitude of the spontaneous contractions of strips from all regions studied. Human detrusor and pig trigone, bladder neck and urethral strips were more sensiitve to VIP than pig detrusor. The response was reversible, reproducible and dose-dependent from 10-9 to 10-6 mol/l. The time to onset of the response was within 1/2 min and the time to maximal relaxation was 2 to 10 min. The response was not affected by selective nerve poisoning with tetrodotoxin, .beta.-adrenergic blockade with propranolol or prostaglandin synthesis blockade with ketoprofen. Vasoactive intestinal polypeptide did not prevent prostaglandin E2 activity on the musculature. Contractions evoked by transmural electric field stimulation or pharmacologically with carbachol, noradrenaline [norepinephrine], substance P and prostaglandin F2.alpha. were equally reduced by VIP 10-7 mol/l.