Identification of stage-specific polypeptides synthesized during murine preimplantation development.
- 1 July 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (7) , 3332-3336
- https://doi.org/10.1073/pnas.75.7.3332
Abstract
[35S]Methionine-labeled extracts of mouse ova and preimplantation embryos were analyzed by 2 dimensional polyacrylamide gel electrophoresis. Of the 400-600 molecular species that were resolved as distinct spots on autoradiograms of gels for every stage of development from unfertilized eggs to early blastocysts, particular attention was paid to the identification of 36 of these proteins, each of which is expressed only for a portion of the period under investigation. These molecules are referred to as stage-specific polypeptides, and they are biochemical markers of early embryonic development and differentiation.Keywords
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