Binding of the ligand [3H]MK-801 to the MK-801 binding site of the N-methyl-D-aspartate receptor during experimental encephalopathy from acute liver failure and from acute hyperammonemia in the rabbit
- 1 June 1993
- journal article
- Published by Springer Nature in Metabolic Brain Disease
- Vol. 8 (2) , 81-94
- https://doi.org/10.1007/bf00996891
Abstract
Binding of the ligand [3H]MK-801 to the MK-801 binding site of the N-methyl-D-aspartate (NMDA) receptor population on brain homogenates in rabbits was studied during experimental encephalopathy from acute liver failure and from acute hyperammonemia in the rabbit. Homogenates were prepared from brain cortex, hippocampus and striatum. Hepatic encephalopathy was induced by a two-stage liver devascularization procedure and acute hyperammonemia by a prolonged ammonium-acetate infusion; rabbits receiving a sodium-potassium-acetate infusion served as controls. In these animal models extracellular brain glutamate levels are known to be elevated. However no significant alterations in the number nor the affinity of the MK-801 binding sites of the NMDA receptors were found during acute liver failure and acute hyperammonemia. These findings suggest that the NMDA receptor population remains unaltered in experimental encephalopathy from acute liver failure and acute hyperammonemia, despite alterations in extracellular brain glutamate levels.Keywords
This publication has 49 references indexed in Scilit:
- Extracellular brain glutamate during acute liver failure and during acute hyperammonemia simulating acute liver failure: An experimental study based on in vivo brain dialysisJournal of Hepatology, 1994
- Encephalopathy from acute liver failure and from acute hyperammonemia in the rabbit. A clinical and biochemical studyLiver International, 1994
- Monitoring of Neurotransmitter Amino Acids by Means of An Indwelling Cisterna Magna Catheter: A Comparison of Two Rodent Models of Fulminant Liver FailureHepatology, 1992
- Alterations in Cortical [3H]Kainate and α‐[3H]Amino‐3‐Hydroxy‐5‐Methyl‐4‐Isoxazolepropionic Acid Binding in a Spontaneous Canine Model of Chronic Hepatic EncephalopathyJournal of Neurochemistry, 1991
- Amino acid neurotransmitters and their receptors in the brain synaptosomes of acute hepatic failure ratsBiochemical Medicine and Metabolic Biology, 1988
- Mode of binding of [3H]dibenzocycloalkenimine (MK‐801) to the N‐methyl‐D‐aspartate (NMDA) receptor and its therapeutic implicationFEBS Letters, 1988
- Excitatory and inhibitory amino acid neurotransmitters and ammonia metabolism in hepatic failure ratsZeitschrift für Die Gesamte Experimentelle Medizin, 1985
- Glutamic acid and glutamine levels in serum and cerebrospinal fluid in hepatic encephalopathyBiochemical Medicine, 1984
- The Release and Neosynthesis of Glutamic Acid Are Increased in Experimental Models of Hepatic EncephalopathyJournal of Neurochemistry, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976