HOG CHOLERA .4. DETECTION OF VIRUS IN TISSUE CULTURE PREPARATIONS BY FLUORESCENT ANTIBODY TECHNIQUE

  • 1 January 1965
    • journal article
    • research article
    • Vol. 29  (9) , 234-+
Abstract
The fluorescent-antibody technique was employed for detection of hog cholera virus in tissue cultures inoculated with spleens of infected animals. As controls, cultures were also inoculated with material from normal swine and from those infected with other agents. In the 1st series 70 of 73 infected spleens, or 97%, were detected. There were no false positive reactions among the controls. Results obtained with the second series of pigs showed that spleens collected during advanced stages of the disease were more satisfacory specimens than those collected earlier during the high temperature phase of infection. Findings with the 3rd series of older swine indicated that their spleens were less satisfactory as a source of virus than those from young pigs. Tissues from freshly killed animals provided better specimen material than those from animals which had died.