Foliar application of molybdenum in common beans. I. Nitrogenase and reductase activities in a soil of high fertility

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Abstract
Although common bean (Phaseolus vulgaris L.) has a good potential for N2 fixation, poor nodulation following inoculation, principally under field conditions, has led to increased nitrogen (N) fertilizer use in this crop. In the face of the negative environmental effects of N fertilizer, alternative methods have been studied to minimize the amount to be applied. In this sense, foliar application of molybdenum (Mo) has been cited as a promising method. Several papers show that high bean yields (1,500–2,500 kg ha‐1), may be obtained in the southeasten region of Brazil, when there is an application of N as side dressing or Mo spray 25 days after plant emergence. A field experiment was carried out to verify the effect of Mo foliar application on nitrogenase and nitrate reductase activities and on bean yield. Treatments included Rhizobium inoculation (with and without), foliar application of Mo (0 and 40 g ha‐1), N at planting (0 and 20 kg ha‐1) and N applied as side dressing (0 and 30 kg ha‐1). Molybdenum and N as side dressing were used 25 days after plant emergence. Molybdenum increased greatly the nitrogenase activity and extended the period of high nitrate reductase activity, with a consequent increase in total shoot N. Increase of nitrogenase activity did not depend on inoculation, showing that soil native rhizobia may increase in effectiveness when appropriately handled. Bean yield did not differ significantly when fertilized with either Mo or N as side dressing.