Recombinant human retinoic acid receptor α
- 3 March 1992
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 204 (3) , 1141-1148
- https://doi.org/10.1111/j.1432-1033.1992.tb16739.x
Abstract
The human retinoic acid receptor alpha was expressed in Escherichia coli. The recombinant protein was found to be very unstable in several E. coli strains, probably due to proteolysis. Conditions were established to obtain reasonable amounts of active protein for ligand and DNA binding studies. The recombinant receptor showed the expected DNA binding activities in gel-retardation assays. Ligand binding properties were measured in a charcoal absorption assay. The dissociation constant for highly specific bound retinoic acid was found to be 0.67 nM. The affinity of several synthetic retinoids to the recombinant protein was determined and compared to their biological activity. Some of the values presented here differ significantly from those published earlier for the receptor or its isolated hormone-binding domain.Keywords
This publication has 45 references indexed in Scilit:
- Temperature-mediated regulation and downstream inducible selection for controlling gene expression from the bacteriophage λ pL promoterGene, 1990
- Effect of structural modifications in the C7-C11 region of the retinoid skeleton on biological activity in a series of aromatic retinoidsJournal of Medicinal Chemistry, 1989
- Nuclear receptors enhance our understanding of transcription regulationTrends in Genetics, 1988
- A new retinoic acid receptor identified from a hepatocellular carcinomaNature, 1988
- Identification of a second human retinoic acid receptorNature, 1988
- A human retinoic acid receptor which belongs to the family of nuclear receptorsNature, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Synthesis and pharmacological activity of 6-[(E)-2-(2,6,6-trimethyl-1-cyclohexen-1-yl)ethen-1-yl)]- and 6-(1,2,3,4-tetrahydro-1,1,4,4-tetramethyl-6-naphthyl)-2-naphthalenecarboxylic acidsJournal of Medicinal Chemistry, 1983
- The induction of differentiation in teratocarcinoma stem cells by retinoic acidCell, 1978
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949