Some Observations on Protein Extraction from Sweet Potato Roots with Black Rot

Open Access
Publisher Website
Google Scholar
Abstract
Comparison of protein content, phosphatase activity and peroxidase activity was made using extracts prepared with and without isoascorbate from sweet potato roots infected by the black rot pathogen. There was no noticeable difference between the two kinds of extracts. Immunological results indicated that some proteins were modified when isoascorbate was omitted from the extraction medium. After SEPHADEX chromatography, protein content and peroxidase activity did not change appreciably despite the presence or absence of isoascorbate. Acid phosphatase activity was strongly decreased in the absence of isoascorbate. It was concluded that acid phosphatase formed a complex with the oxidation products from polyphenols formed by polyphenol oxidase. SEPHADEX sieving was found to be a very useful method to separate proteins quickly from polyphenols, because the latter were strongly adsorbed on SEPHADEX.

This publication has 0 references indexed in Scilit: