Restriction cleavage map of SP01 DNA: general location of early, middle, and late genes

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Abstract
A detailed restriction endonuclease map for the genome of Bacillus subtilis phage SPO1 is presented. Sites of cleavage for the restriction enzymes BhlII, EcoRI*, HaeIII, and SalI were determined. This physical map showed that SPO1 DNA was 140 kilobases in length and contained a repeated sequence of 12.4 kilobases at its termini. Combined with previously published information, it was possible to identify the general locations of genes expressed at early, middle or late times in the phage lytic cycle. In particular, early genes were largely clustered in the terminal repeats, whereas a major cluster of late genes was located in the left-central portion of the genome.