Class II tubulin, the major brain β tubulin isotype is polyglutamylated on glutamic acid residue 435

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Abstract
Protein sequencing shows that porcine brain tubulin retains the N‐terminal sequences of α and β tubulin after a mild treatment with subtilisin, C‐terminal peptides released by subtilisin were purified and characterized by automated Edman degradation and mass spectrometry. We confirm the polyglutamylation of α tubulin on glutamic acid residue 445 reported by others and show in addition that class 11 β tubulin, the major β tubulin isotype of adult brain, is also polyglutamylated. The substitution is restricted to glutamic acid residue 435. Thus all major tubulin isotypes of adult brain are subjected to polyglutamylation.