The Production of Anti-Idiotypic Antibodies to BALB/c Plasmacytoma Globulins in BALB/c Mice

Open Access
Publisher Website
Google Scholar
Abstract
Slater, Ward and Kunkel (1) were the first to draw attention to the individual antigenic characteristics of myeloma globulins. Later it was determined that these globulins have the immunochemical properties of idiotypic antibodies with specific antigenic groupings in the variable portion of the Fab fragment (2–4). Antibodies to these determinants have been developed by prolonged immunization of rabbits with myeloma globulins and absorption of these antibodies with large amounts of normal γ globulin (2, 3), thus removing antibodies to the non-idiotypic components of the molecules. Absorption would not be required if immunization could be accomplished in a recipient with the same allotype as the donor. The following report deals with the production of anti-idiotypic antibodies to BALB/c plasmacytoma globulins in normal BALB/c mice. The presence of such antibodies was demonstrated by hemagglutination, hemagglutination inhibition, and also in some instances by immunodiffusion. Materials and Methods.

This publication has 0 references indexed in Scilit: