Phosphorylation of Hic‐5 at tyrosine 60 by CAKβ and Fyn
- 26 May 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 474 (2-3) , 179-183
- https://doi.org/10.1016/s0014-5793(00)01597-0
Abstract
Hic‐5 is a CAKβ‐binding protein localized at focal adhesions. Here we show that overexpression of CAKβ or Fyn, but not FAK, enhanced the tyrosine phosphorylation of coexpressed Hic‐5 in COS‐7 cells. These phosphorylations were further augmented by stimulating cells with osmotic stress. The Y60F mutant of Hic‐5 was not phosphorylated, and Hic‐5 phosphorylated on tyrosine 60 was bound specifically to the SH2 domain of Csk. Coexpression experiments revealed that the phosphorylation of Hic‐5 by CAKβ required the kinase activation of CAKβ and binding of Hic‐5 by CAKβ. Specific phosphorylation of Hic‐5 by CAKβ and Fyn may activate a signaling pathway mediated by Hic‐5.Keywords
This publication has 35 references indexed in Scilit:
- Cell Adhesion Kinase β Forms a Complex with a New Member, Hic-5, of Proteins Localized at Focal AdhesionsJournal of Biological Chemistry, 1998
- Activation of Protein-tyrosine Kinase Pyk2 Is Downstream of Syk in FcεRI SignalingPublished by Elsevier ,1997
- Differential Signaling by the Focal Adhesion Kinase and Cell Adhesion Kinase βJournal of Biological Chemistry, 1997
- The Related Adhesion Focal Tyrosine Kinase Differentially Phosphorylates p130Cas and the Cas-like Protein, p105HEF1Journal of Biological Chemistry, 1997
- RAFTK, a Novel Member of the Focal Adhesion Kinase Family, Is Phosphorylated and Associates with Signaling Molecules upon Activation of Mature T LymphocytesThe Journal of Experimental Medicine, 1997
- The Related Adhesion Focal Tyrosine Kinase Is Tyrosine-phosphorylated after β1-Integrin Stimulation in B Cells and Binds to p130casJournal of Biological Chemistry, 1997
- Activation of a Novel Calcium-dependent Protein-tyrosine KinasePublished by Elsevier ,1996
- Identification and Characterization of a Novel Related Adhesion Focal Tyrosine Kinase (RAFTK) from Megakaryocytes and BrainJournal of Biological Chemistry, 1995
- Cloning and Characterization of Cell Adhesion Kinase β, a Novel Protein-tyrosine Kinase of the Focal Adhesion Kinase SubfamilyJournal of Biological Chemistry, 1995
- Protein tyrosine kinase PYK2 involved in Ca2+-induced regulation of ion channel and MAP kinase functionsNature, 1995