Separation of 5' -Mono, Di- and Triphosphates of 1- -D-Arabinofuranosylcytosine and of 1- -D-Arabinofuranosyluracil by Thin-Layer and High Pressure Liquid Chromatography
- 1 July 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 14 (7) , 331-333
- https://doi.org/10.1093/chromsci/14.7.331
Abstract
The separation of all the 5′-mono-, di- and triphosphates of ara-C and ara-U by thin-layer (TLC) and high pressure liquid chromatography (HPLC) is described. Whereas for the TLC two different systems are necessary, HPLC allows a rapid separation of all the nucleotides with high resolution, reproducibility, and sensitivity within 15 minutes. An example of an HPLC separation of ara-C and ara-U nucleotides derived from a biological sample is presented.This publication has 4 references indexed in Scilit:
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- CHEMOTHERAPY OF LEUKEMIA L1210 IN MICE WITH 1-BETA-D-ARABINOFURANOSYLCYTOSINE HYDROCHLORIDE .I. INFLUENCE OF TREATMENT SCHEDULES1966
- Comparative studies of leukemic cells sensitive and resistant to cytosine arabinosideBiochemical Pharmacology, 1965
- Megaloblastosis Produced by a Cytosine AntagonistBlood, 1963