Erythropoietin. I. Analysis of Radioiron Assay in Fasted Rats, Using Cobalt as a Reference Standard.
- 1 January 1960
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 103 (1) , 43-46
- https://doi.org/10.3181/00379727-103-25406
Abstract
Radioiron assay of erythropoietin has been analyzed and for purposes of inter-lab comparison of erythropoietic activity. the response to 5 μmoles of cobalt, given by subcutaneous injections, is suggested to represent 1 unit of activity. A house standard of partially purified sheep erythropoietin, having a potency of 1 unit/mg, has been used to establish a dose-response curve up to 22.4 units rat. In this range the curve shows an increasing slope to 11.3 units/rat. On the basis of this assay, the potency of several fractions of sheep erythropoietin has been given.Keywords
This publication has 3 references indexed in Scilit:
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- Studies on ErythropoiesisBlood, 1958
- Studies on Erythropoiesis. III. Factors Controlling Erythropoietin ProductionExperimental Biology and Medicine, 1957