Photoaffinity Labeling of Different Benzodiazepine Receptors at Physiological Temperature
- 1 December 1984
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 43 (6) , 1745-1748
- https://doi.org/10.1111/j.1471-4159.1984.tb06103.x
Abstract
Irreversible labeling of benzodiazepine receptors in membranes from cerebellum or hippocampus was compared at 0°C or 37°C using [3H]flunitrazepam as a photoaffinity ligand. [3H]Flunitrazepam reproducibly and irreversibly labeled mainly one protein (P51) in cerebellum and at least two proteins (P51 and P55) in hippocampus at both temperatures. Differential inhibition at 37°C of irreversible [3H]flunitrazepam binding to the individual proteins by several selective benzodiazepine receptor ligands supports the hypothesis that P51 and P55 are associated with different benzodiazepine receptors.Keywords
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