Immunofluorescent Identification of Cells Synthesizing Human Growth Hormone in Culture
- 1 January 1971
- journal article
- research article
- Published by Georg Thieme Verlag KG in Hormone and Metabolic Research
- Vol. 3 (05) , 309-313
- https://doi.org/10.1055/s-0028-1094132
Abstract
Cells have been established in culture from human pituitary tissue obtained from an acromegalic patient. High levels of immunoreactive growth hormone were released into culture media during the first six weeks of culture. Cells transformed with SV40 virus continued to produce hormone after several subcultures over eight months. The cells synthesizing growth hormone were identified by immunofluorescence. Evidence is presented that the cells have incorporated 14C-leucine into growth hormone synthesized and released into the medium. The growth hormone appears to be secreted in a monomeric form. 1 This work was supported by the National Health and Medical Research Council of Australia, the Victorian Anti-Cancer Council and the Joseph Hermann Trust (University of Melbourne). We are grateful to Professor R.C. Nairn for advice and to Mr. D. Quinn for excellent technical assistance.Keywords
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