Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation
Open Access
- 26 May 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 408 (3) , 297-300
- https://doi.org/10.1016/s0014-5793(97)00447-x
Abstract
We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4‐hydroxyalkenals (4‐HDA) concentrations following the induction of lipid peroxidation with and without pre‐incubation with melatonin (1 μM–3 mM). Membrane rigidity increased during induced lipid peroxidation while melatonin reduced in a concentration‐dependent manner both membrane rigidity and MDA+4‐HDA generation. Melatonin's protective effect may relate to its known ability to scavenge free radicals and function as an antioxidant.Keywords
This publication has 37 references indexed in Scilit:
- Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellumLife Sciences, 1994
- Melatonin: A peroxyl radical scavenger more effective than vitamin ELife Sciences, 1994
- Iron/ascorbate-induced lipid peroxidation changes membrane fluidity and muscarinic cholinergic receptor binding in rat frontal cortexNeurochemistry International, 1993
- MEMBRANE DAMAGE INDUCED IN CULTURED HUMAN SKIN FIBROBLASTS BY UVA IRRADIATIONPhotochemistry and Photobiology, 1993
- Initiation of lipid peroxidation in biological systemsC R C Critical Reviews in Food Science and Nutrition, 1987
- Differential effect of lipid peroxidation on membrane fluidity as determined by electron spin resonance probesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983
- Microsomal lipid peroxidation causes an increase in the order of the membrane lipid domainFEBS Letters, 1982
- Interaction of vitamin E with saturated phospholipid bilayersBiochemical and Biophysical Research Communications, 1982
- The increase of phospholipid bilayer rigidity after lipid peroxidationFEBS Letters, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976