Structural domains of ribosomal protein S8 and their relationship to ribosomal RNA binding
- 29 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 149 (2) , 328-333
- https://doi.org/10.1016/0014-5793(82)81125-3
Abstract
Escherichia coli ribosomal protein S8 has been subjected to mild proteolytic digestion in order to search for structural domains within the protein [1]. A characteristic fragment produced in high yield after chymotrypsin treatment has been located with the protein sequence. Circular dichroism has shown this domain to be rich in α helix. However, the fragment loses its ability to bind to 16 S rRNA as does a similar fragment produced by trypsin cleavage. The intact protein is required for rRNA binding and is highly protected against proteolytic digestion when bound to the RNA.Keywords
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