An interleukin 4-induced DNase I hypersensitive site indicates opening of the γ1 switch region prior to switch recombination
- 1 November 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 1 (6) , 570-575
- https://doi.org/10.1093/intimm/1.6.570
Abstract
Interleukin 4 (IL4) directs class switch recombination to the γ1 switch region (sγ1) in mitogenactivated murine B lymphocytes. The finding that transcription of the unrearranged sγ1 and its 5′ flanking region is induced by IL4 prior to switch recombination had argued for the idea that IL4 promotes sμ/γ1 switch recombination by selectively inducing accessibility of the γ1 switch region for a general switch recombinase. Here further evidence for this model is provided by showing that withln γ1 region in activated murine B cells. This change in chromatin structure most likely reflects the IL4-responsive binding of regulatory proteins within or in the vicinity of the HS site. The result provides a candidate sequence for an IL4-dependent enhancer/promoter of transcription of sγ1.Keywords
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