Biosynthesis of glycoproteins inCandida albicans: Processing of a Man6-GlcNAc2-Asn oligosaccharide by membrane fractions
- 1 June 1993
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 110 (1) , 121-125
- https://doi.org/10.1111/j.1574-6968.1993.tb06305.x
Abstract
When a M6-GlcNAc2-Asn oligosaccharide isolated from ovalbumin was incubated with a membrane fraction from Candida albicans and GDP-[14C]Man only one set of low molecular mass products was obtained, which contained 7, 8, 9 and 10 mannose residues in a ratio of 13:6:3:1, as deduced from fast atomic bombardment mass spectrometry analysis. When a mixture of these products was reincubated with a fresh membrane preparation and cold GDP-Man, no further mannose incorporation was observed, indicating that these compounds are not recognized as substrates by the glycan processing system in this organism. Results are discussed in terms of the early glycan processing steps in C. albicans.Keywords
This publication has 4 references indexed in Scilit:
- Biosynthesis of glycoproteins inCandida albicans: Biochemical characterization of a soluble α-mannosidaseFEMS Microbiology Letters, 1993
- Localization of .alpha.1 .fwdarw. 3-linked mannoses in the N-linked oligosaccharides of Saccharomyces cerevisiae mnn mutantsBiochemistry, 1990
- Regulation of the protein glycosylation pathway in yeast: structural control of N-linked oligosaccharide elongation.Proceedings of the National Academy of Sciences, 1987
- Neoglycoproteins: preparation and properties of complexes of biotinylated asparagine-oligosaccharides with avidin and streptavidinBiochemistry, 1986