Amperometric Immunosensors Based on Protein A Coupled Polyaniline−Perfluorosulfonated Ionomer Composite Electrodes
- 6 May 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 72 (13) , 2925-2929
- https://doi.org/10.1021/ac9914317
Abstract
A very sensitive immunosensor based on polyaniline/Nafion/protein A (PA/NF/PrA) composite electrodes has been developed for the amperometric immunoanalysis with urease-labeled immunoreagents. The use of urease conjugated goat anti-RIgG (GaRIgG-Ur) as the labeled antibody and urea as the substrate with an amperometric detection at −200 mV (vs Ag/AgCl) resulted in a dynamic range of 50−2000 ng mL-1 and a low detection limit of 10 ng/mL (64 pM) for the immunoanalysis of rabbit immunoglobulin G (RIgG). Because of the special affinity between protein A and RIgG, the PA/NF/PrA electrode can be regenerated repetitively by changing the pH of the buffer solutions. Characteristics of the PA/NF/PrA/RIgG immunosensor and optimal conditions for the competitive immunoanalysis of RIgG with FIA were studied.Keywords
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