A method for determining the concentration of ubiquinone in mitochondrial preparations

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Abstract
A method for determination of the ubiquinone concentration in small amounts of mitochondrial preparations is described. Ubiquinone occurs in animal and plant mitochondria in relatively high concentrations. In heart muscle preparations, for example, the concentration, on a molar basis, is 4 to 5 times those of the individual cytochromes. A "bound" form of ubiquinone appears to exist in phosphate-buffered heart muscle preparations. This bound form, which amounts to about 25% of the total ubiquinone, can be extracted only after treatment of the preparation with alkali.