Caries-like Lesions in Rat Calculus

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Abstract
Rat caries were studied by an incipient lesion method in which hypomineralized enamel and dentin are stained with 1% AgNO, after which the soft tissue was removed and the molars are sectioned longitudinally at the mid-line. The sectioned molars are then mounted on microscope slides for grading. Most teeth have no calculus, but calculus is found on the mesial surfaces of some (about 20%) of the maxillary 1st molars. This calculus either does not take up the AgNO3 stain or stains a light brown. In calculus forming animals, AgNO3 staining areas and gross cavitation of the calculus were seen indicating that pre-formed calculus may undergo caries-like attack to the rat.

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