The Entire Population ofThermus thermophilusCells Is Always Competent at Any Growth Phase

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Abstract
Thermus thermophilus mutants carrying unlinked double auxotrophic markers were transformed with wild type chromosomal DNA at various growth phases. The percentages of competent cells in the total population were calculated based on the results of transformation efficiencies for single or double markers. It was concluded that all the T. thermophilus cells at any growth phase were competent.

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