Multiple Nucleotide Substitutions in the Neisseria meningitidis Serogroup C ctrA Gene Cause False-Negative Detection by Real-Time PCR
- 1 August 2010
- journal article
- case report
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (8) , 3016-3018
- https://doi.org/10.1128/jcm.00103-10
Abstract
Two strains of Neisseria meningitidis serogroup C with disparate sequences of ctrA were isolated. The nucleotide substitutions did not alter the corresponding protein sequences, but they impeded the detection of these meningococcal isolates by real-time PCR.Keywords
This publication has 8 references indexed in Scilit:
- Real-time polymerase chain reaction detection of Neisseria meningitidis in formalin-fixed tissues from sudden deathsDiagnostic Microbiology and Infectious Disease, 2008
- Standardized nonculture techniques recommended for European reference laboratoriesFEMS Microbiology Reviews, 2007
- Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidisJournal of Clinical Microbiology, 2005
- PCR-Based Assay for Detection of Neisseria meningitidis Capsular Serogroups 29E, X, and ZJournal of Clinical Microbiology, 2004
- Use of Real-Time PCR To Resolve Slide Agglutination Discrepancies in Serogroup Identification of Neisseria meningitidisJournal of Clinical Microbiology, 2004
- Evaluation of non-culture diagnosis of invasive meningococcal disease by polymerase chain reaction (PCR)FEMS Immunology & Medical Microbiology, 2003
- Simultaneous Detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in Suspected Cases of Meningitis and Septicemia Using Real-Time PCRJournal of Clinical Microbiology, 2001
- Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNAFEMS Immunology & Medical Microbiology, 2000