The perifused isolated left atrium: a preparation for studies on sugar transport in the myocardium

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Abstract
A procedure is described for the in vitro perifusion of the rat left atrium by recirculating medium through the atrial cavity and around the tissue, and for making the left atrium completely quiescent using surgical methods. The main advantage of this preparation is that it provides direct data on metabolic and transport processes in resting myocardium. Its use is illustrated by data on the time course, concentration dependence, insulin sensitivity and chemical specificity of the sugar transport system; in all these respects the resting left atrium resembles resting skeletal muscle.