A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus
Top Cited Papers
- 9 June 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 312 (5779) , 1526-1530
- https://doi.org/10.1126/science.1128393
Abstract
Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus. The apparatus assembled in discrete subcellular locations and exported Hcp1, a hexameric protein that forms rings with a 40 angstrom internal diameter. Regulatory patterns of HSI-I suggested that the apparatus functions during chronic infections. We detected Hcp1 in pulmonary secretions of cystic fibrosis (CF) patients and Hcp1-specific antibodies in their sera. Thus, HSI-I likely contributes to the pathogenesis of P. aeruginosa in CF patients. HSI-I–related loci are widely distributed among bacterial pathogens and may play a general role in mediating host interactions.Keywords
This publication has 19 references indexed in Scilit:
- Keeping Their Options Open: Acute versus Persistent InfectionsJournal of Bacteriology, 2006
- Chaperone release and unfolding of substrates in type III secretionNature, 2005
- sciS , an icmF Homolog in Salmonella enterica Serovar Typhimurium, Limits Intracellular Replication and Decreases VirulenceInfection and Immunity, 2005
- The Genetic Basis for the Commitment to Chronic versus Acute Infection in Pseudomonas aeruginosaMolecular Cell, 2004
- Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpBCell, 2004
- IcmF and DotU Are Required for Optimal Effector Translocation and Trafficking of the Legionella pneumophila VacuoleInfection and Immunity, 2004
- Unveiling Molecular Scaffolds of the Type IV Secretion SystemJournal of Bacteriology, 2004
- In vivo functional genomics of Pseudomonas aeruginosa for high‐throughput screening of new virulence factors and antibacterial targetsEnvironmental Microbiology, 2003
- Roles of Individual Domains and Conserved Motifs of the AAA+ Chaperone ClpB in Oligomerization, ATP Hydrolysis, and Chaperone ActivityJournal of Biological Chemistry, 2003
- Infection-Blocking Genes of a Symbiotic Rhizobium leguminosarum Strain That Are Involved in Temperature-Dependent Protein SecretionMolecular Plant-Microbe Interactions®, 2003