Sequence determination of multiply charged peptide molecular ions by electrospray–ionization tandem mass spectrometry
- 1 May 1989
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 3 (5) , 160-164
- https://doi.org/10.1002/rcm.1290030512
Abstract
Electrospray ionization generates multiply charged peptide molecular ions which are efficiently dissociated by collision with a neutral gas to provide sequence‐specific daughter ions. The scope for application of this phenomenon is illustrated by the nearly complete sequencing of melittin (relative molecular mass (Mr) 2846.5) employing a tandem quadrupole mass spectrometer having a mass limit of m/z 1400.Keywords
This publication has 24 references indexed in Scilit:
- Microbore high performance liquid chromatography–ion spray mass spectrometry for the determination of peptidesJournal of Microcolumn Separations, 1989
- Collisional effects on the charge distribution of ions from large molecules, formed by electrospray‐ionization mass spectrometryRapid Communications in Mass Spectrometry, 1988
- Solvent effects on the charge distribution observed with electrospray ionization-mass spectrometry of large moleculesJournal of Mass Spectrometry, 1988
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988
- FAB and tandem mass spectrometry for endorphin- and ACTH peptides of molecular weight to 2000Journal of Mass Spectrometry, 1988
- Ion spray interface for combined liquid chromatography/atmospheric pressure ionization mass spectrometryAnalytical Chemistry, 1987
- The primary structure of thioredoxin from Chromatium vinosum determined by high-performance tandem mass spectrometryBiochemistry, 1987
- Electrospray ion source. Another variation on the free-jet themeThe Journal of Physical Chemistry, 1984
- Energy transfer in the collision induced decomposition of peptide ions formed by field desorptionJournal of Mass Spectrometry, 1984
- Intrinsic chemistry of peptide ions: Field desorption and collision-induced decomposition of bradykininAustralian Journal of Chemistry, 1984