Abstract
The resting cells aerobically accumulate glutamate from acetate in the presence of ammonium ions and incorporate C14O2 exclusively to alpha-carboxyl C of the glutamate molecule. The cell-free extract catalyzes the acetyl-Co A formation as is evidenced by the consecutive formation of oxaloacetate and glyoxylate in the presence of citrate and malate, respectively. This extract also contains malic dehydrogenase and malic enzyme. A metabolic pathway of the cell is presented as consisting of the usual tricarboxylic acid cycle and a by-pass from isocitrate via glyoxylate to malate(acetyl-Co A requiring).