EFFECT OF STORAGE ON NORMAL NEUTROPHILS COLLECTED BY DISCONTINUOUS-FLOW CENTRIFUGATION LEUKAPHERESIS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 50  (6) , 1145-1150
Abstract
Storage limits for neutrophils [human] collected by discontinuous-flow centrifugation and stored at 4-6.degree. C were determined. The parameters studied were total leukocyte and absolute cell counts, viability measured by dye exclusion, morphology, percentage phagocytic neutrophils, number of Candida organisms ingested per phagocytic neutrophil, candidacidal activity by differential staining and chemotaxis under agarose. There was a progressive loss of neutrophils on storage that was statistically significant by 48 h. Phagocytosis was the best preserved function. Microbial killing measured by candidacidal activity was less well preserved. Chemotaxis was the most poorly maintained parameter. There was milk impairment at 24 h and a severe functional loss at 48 h. The 1st functions lost on storage are apparently the most highly integrated, i.e., chemotaxis, followed by microbial killing and then phagocytosis; assuming that these functional losses are ireversible, storage of normal neutrophils used for transfusion should be limited to approximately 24 h because a severe defect in migration occurs between the 1st and 2nd days.

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